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Genetic Engineering Techniques module quiz: restriction enzymes, cloning, PCR, electrophoresis and profiling (SEAB O-Level Applied Subject) quiz

14questions. Pick an answer and you'll see why right away.

  1. What does a restriction enzyme do?

  2. What is the role of DNA ligase in making recombinant DNA?

  3. Why is the same restriction enzyme used to cut both the gene and the plasmid?

  4. What is a 'sticky end' produced by some restriction enzymes?

  5. During transformation, how do host bacteria take up a recombinant plasmid?

  6. What is the purpose of cloning a transformed bacterial cell?

  7. What does PCR achieve?

  8. Which is the correct order of the three steps in one PCR cycle?

  9. Starting from one copy and assuming the amount doubles each cycle, how many copies are there after 4 PCR cycles?

  10. In gel electrophoresis, why does DNA move towards the positive electrode?

  11. In a gel, which DNA fragments travel furthest from the wells?

  12. What is the main difference between DNA sequencing and genetic profiling?

  13. Genetic profiling (DNA fingerprinting) is most commonly used to:

  14. Which technique provides the read-out (banding pattern) used in both restriction analysis and genetic profiling?