Which two enzymes are used to insert the insulin gene into a plasmid?

A restriction enzyme and DNA ligase

Which is an advantage of recombinant human insulin over insulin extracted from animals?

It matches human insulin exactly, so it causes fewer allergic reactions

It trains the body's own immune system to make antibodies and memory cells

A single type of antibody, made in the laboratory, that targets one specific molecule

Vaccines train the body's own immune response while monoclonal antibodies are ready-made and act directly

To amplify and detect a pathogen's DNA, even from a tiny sample

Checking a person's DNA for disease-causing alleles, sometimes before symptoms appear

Because the results may reveal disease risk and raise issues of privacy, consent and how the information is used

Adding a working copy of a faulty gene, usually delivered into cells by a vector

An unspecialised cell that can divide and develop into different cell types

They can be technically difficult, costly, carry safety risks, and some stem cell sources raise ethical concerns

Restriction enzymes, ligase, transformation and bioreactor culture

14questions. Pick an answer and you'll see why right away.

In producing recombinant insulin, what is inserted into the plasmid?
Which two enzymes are used to insert the insulin gene into a plasmid?
Where are the transformed bacteria grown to produce insulin on a large scale?
Which is an advantage of recombinant human insulin over insulin extracted from animals?
How does a vaccine protect a person from a disease?
What is a monoclonal antibody?
Which is a key difference between vaccines and monoclonal antibodies?
How can PCR be used in diagnosis?
What is genetic screening?
Why does genetic screening raise ethical questions?
What is the basic principle of gene therapy?
What is a stem cell?
Which is a limitation of gene therapy and stem cell treatments?
Which earlier biotechnology techniques are combined to produce recombinant insulin?