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Experimental Chemistry and Separation Techniques

Quick questions on Paper chromatography explained: O-Level Chemistry

5short Q&A pairs drawn directly from our worked dot-point answer. For full context and worked exam questions, read the parent dot-point page.

What is reading a chromatogram?
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A finished chromatogram tells you two things directly:
What are locating colourless substances?
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If the substances are colourless (such as amino acids or sugars), the separated spots are invisible. They are revealed by spraying the paper with a locating agent that reacts to give a colour, or by viewing under ultraviolet light if the spots fluoresce.
What is q1?
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A mixture produces four spots on a chromatogram. State how many substances it contains. [1 mark]
What is q2?
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A spot moves 3.6 cm3.6\ \text{cm} while the solvent front moves 9.0 cm9.0\ \text{cm}. Calculate its RfR_f value. [2 marks]
What is q3?
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Explain why the start line on a chromatogram is drawn in pencil and not in ink. [2 marks]

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