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Cells and Microorganisms

Quick questions on Aseptic technique and sterilisation: O-Level Cells and Microorganisms

5short Q&A pairs drawn directly from our worked dot-point answer. For full context and worked exam questions, read the parent dot-point page.

What is aseptic technique?
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Aseptic technique is the set of careful working practices that keep contaminants out. The key steps are:
What is methods of sterilisation?
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Sterilisation kills or removes all microorganisms from equipment and media before use:
What is q1?
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State two ways a culture could become contaminated. [2 marks]
What is q2?
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Explain why an inoculating loop is flamed before and after transferring bacteria. [2 marks]
What is q3?
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Name the method used to sterilise nutrient media and glassware, and state how it works. [2 marks]

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