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Genetic Engineering Techniques
Quick questions on The polymerase chain reaction: O-Level Genetic Engineering Techniques
5short Q&A pairs drawn directly from our worked dot-point answer. For full context and worked exam questions, read the parent dot-point page.
What is the three steps of a cycle?Show answer
One cycle of PCR has three steps, set by temperature:
What is doubling each cycle?Show answer
At the end of each cycle, every target molecule has become two. So the number of copies doubles each cycle. Repeating the cycle around 30 times gives millions of copies.
What is q1?Show answer
Name the three steps of one PCR cycle in order. [3 marks]
What is q2?Show answer
State the role of the primers in PCR. [1 mark]
What is q3?Show answer
Starting from one copy, how many copies are there after cycles? [1 mark]
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