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Genetic Engineering Techniques
Quick questions on Restriction enzymes and ligase: O-Level Genetic Engineering Techniques
6short Q&A pairs drawn directly from our worked dot-point answer. For full context and worked exam questions, read the parent dot-point page.
What are restriction enzymes?Show answer
A restriction enzyme is an enzyme that cuts DNA at a specific base sequence called its recognition site.
What are sticky ends?Show answer
Sticky ends are useful because they are single-stranded and can pair, by complementary base pairing, with any other sticky end made by the same enzyme. This is what lets pieces of DNA from different sources join together.
What is dNA ligase?Show answer
DNA ligase is an enzyme that joins pieces of DNA by sealing the sugar-phosphate backbone. After two sticky ends have paired up, ligase forms the bonds that make the join permanent.
What is q1?Show answer
State what a restriction enzyme does to DNA. [1 mark]
What is q2?Show answer
Explain why the gene and the plasmid must be cut with the same restriction enzyme. [2 marks]
What is q3?Show answer
State the role of DNA ligase in making recombinant DNA. [1 mark]
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