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Genetic Engineering Techniques

Quick questions on Gene cloning and transformation: O-Level Genetic Engineering Techniques

5short Q&A pairs drawn directly from our worked dot-point answer. For full context and worked exam questions, read the parent dot-point page.

What is transformation?
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Transformation is the process by which bacteria take up the recombinant plasmid from their surroundings.
What are identifying transformed cells with marker genes?
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Because not every bacterium is transformed, plasmids carry a marker gene that makes the successful ones detectable:
What is q1?
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Define transformation as used in genetic engineering. [1 mark]
What is q2?
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Explain how an antibiotic-resistance marker gene is used to identify transformed bacteria. [2 marks]
What is q3?
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State what happens to the gene as the transformed bacteria multiply in a bioreactor. [2 marks]

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