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DNA and Genetic Material
Quick questions on Plasmids and vectors: O-Level DNA and Genetic Material
6short Q&A pairs drawn directly from our worked dot-point answer. For full context and worked exam questions, read the parent dot-point page.
What are plasmids?Show answer
A plasmid is a small, circular piece of DNA found in bacteria, separate from the main bacterial chromosome. Plasmids are ideal vectors because:
What are other vectors?Show answer
Plasmids are not the only vectors:
What is building a recombinant plasmid?Show answer
To insert a gene, the plasmid and the gene are both cut with the same restriction enzyme so they have matching sticky ends. The sticky ends pair up, and DNA ligase seals them, forming a recombinant plasmid that carries the new gene.
What is q1?Show answer
Define the term vector as used in genetic engineering. [1 mark]
What is q2?Show answer
State two reasons plasmids make good vectors. [2 marks]
What is q3?Show answer
Name one type of vector other than a plasmid and state why it can carry genes. [2 marks]
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