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Cell Structure and Organisation
Quick questions on Using the light microscope explained: O-Level Biology
5short Q&A pairs drawn directly from our worked dot-point answer. For full context and worked exam questions, read the parent dot-point page.
What is parts of the light microscope?Show answer
The main parts are the eyepiece lens (which you look through), the objective lenses (of different powers, on a rotating turret), the stage (where the slide sits, held by clips), the focusing knobs (coarse and fine), and the light source or mirror (which lights the specimen from below). Light passes up through the thin specimen and through the two lenses to give a magnified image.
What is the magnification equation?Show answer
The total magnification is the eyepiece magnification multiplied by the objective magnification. The key working equation links three quantities:
What is q1?Show answer
State the equation linking magnification, image size and actual size. [1 mark]
What is q2?Show answer
A structure is wide and is drawn wide. Calculate the magnification. [2 marks]
What is q3?Show answer
Explain why a stain is added when preparing a slide of cells. [2 marks]
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