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Cell Structure and Organisation
Quick questions on Light microscope and magnification: N(A)-Level Biology Cell Structure
6short Q&A pairs drawn directly from our worked dot-point answer. For full context and worked exam questions, read the parent dot-point page.
What is parts of the light microscope?Show answer
A light microscope uses two lenses to make a small object look much bigger. The main parts are the eyepiece lens (at the top, where you look), the objective lenses (the set of lenses near the slide, each a different power), the stage (where the slide sits), the focusing knobs, and a light source or mirror underneath that shines light up through the slide.
What is preparing a simple slide?Show answer
A good slide is thin so that light passes through it. To make a wet-mount slide: place a thin layer of the specimen on a clean glass slide, add a drop of water or a stain (such as iodine for plant cells), and lower a cover slip gently at an angle with a mounting needle. The angle stops air bubbles being trapped, which would block the view.
What is the magnification calculation?Show answer
Magnification tells you how many times larger the image is than the real object. The formula is:
What is q1?Show answer
State the formula used to calculate magnification. [1 mark]
What is q2?Show answer
A cell measures in a drawing made at a magnification of . Calculate the real size of the cell in mm. [2 marks]
What is q3?Show answer
Explain why a cover slip is lowered gently at an angle when preparing a slide. [2 marks]
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